Date Range:
Tuesday, January 1, 2002 to Monday, July 26, 2021
Publication Date:
Thursday, July 29, 2021
Microbial genomic sequence data is available by conducting searches using accession numbers associated with the sequence of interest.
Examples of of searches are:
Entrez search, easily search by single accession number; returns sequence and publication information. Link: http://www.ncbi.nlm.nih.gov/sites/gquery
Batch Entrez, Batch search a series of accession numbers after creating a Fasta-formatted text file; returns links to GenBank, Fasta, Graphics, Population Set and Related Sequences for each accession number.
Link: http://ncbi.nlm.nih.gov/sites/batchentrez
Batch Entrez Fasta-formatted text file should be a single column list of accession numbers with a ".1" added like a file extension. File should have no header line. Go to http://ncbi.nlm.nih.gov/sites/batchentrez, follow the web site directions for using Batch Entrez
Fasta-formatted text file example:
AY836800.1
AY836801.1
AY836802.1
AY836803.1
etc..
To access MG-RAST annotation and raw sequences, the user will need to navigate to this page:
http://www.mg-rast.org/mgmain.html?mgpage=search
and enter the project id listed in the PIE-Microbe-Genomics tab (e.g. mgp84173) into the text box at the upper left of the page adjacent to the "search" button.
The search will return a list of the samples associated with the project and a link to download the annotation, fasta files, and associated metadata.
Data can also be accessed through the API according to the instructions here
http://api-ui.mg-rast.org/api.html
Also available, courtesy of Joe Vineis, is a small git repository that includes some simple instructions for handling the API for beginners:
https://github.com/jvineis/mg_rast_processing
Ongoing research
Version 01: March 6, 2012, updated data and metadata. Used MarcrosExportEML_HTML (working)pie_excel2007.xlsm 2/28/2012 8:18 PM for QA/QC to EML 2.1.0
Version 02: April 20, 2018, data and metadata created to comply with importation to Drupal and LTER PASTA. Used MarcrosExportEML_HTML (working)pie_excel2007_Jan2018.xlsm 1/30/2018 2:24 PM for QA/QC to EML 2.1.0
Version 03: August 13, 2019, edited URL made shorter, data and metadata updates to comply with importation to DEIMS7 and LTER Data Portal. Used MarcrosExportEML_HTML (working)pie_excel2007_Jun2019.xlsm 6/7/19 12:58 PM for QA/QC to EML 2.1.0.
Version 04: August 26, 2019, data and metadata updates to comply with importation to DEIMS7 and LTER Data Portal. Used MarcrosExportEML_HTML (working)pie_excel2007_Jun2019.xlsm 6/7/19 12:58 PM for QA/QC to EML 2.1.0.
Version 05:July 29, 2021, Data and metadata updates. Used MarcrosExportEML_HTML (working)pie_excel2007_Jul2021.xlsm 7/26/2021 9:04 AM for QA/QC to EML 2.1.0.