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Water chemistry including nutrient concentrations for various forms of N, P, C, as well as suspended sediments and light extinction coefficients, are determined from bi-annual or tri-annual nutrient transects from the mouth of the Rowely River estuary to near its head about 6 km upstream. Three sets of grab samples and sonde measurements are taken at lower, mid, and upper stations of the estuary, at stations locations that may vary depending on the tide. These samples are generally collected in Spring, Summer, and Fall to capture conditions through the growing season.