Mummichog (Fundulus heteroclitus) caloric value analysis from various collections along Rowley River tidal creeks associated with long term fertilization experiments, Rowley, MA.


At PIE, mummichog (Fundulus heteroclitus) use the spring-cycle high tides to access the flooded high marsh platform and eat invertebrate prey, coupling the high marsh and aquatic creek food webs by gathering energy produced on the high marsh and making it available to the aquatic food web. Changes in the geomorphology of saltmarsh creek edges greatly influence the survival, biomass, and resource use of mummichog populations. Here, we use bomb calorimetry to assess individual mummichog caloric content per gram of biomass at 4 PIE creeks known to present different geomorphologic patterns in their low marsh zones. These data can be used for the assessment of the impact of low marsh geomorphology on mummichog caloric content and energy production in PIE food webs. These data were included as part of two studies “Habitat decoupling via saltmarsh creek geomorphology alters connection between spatially-coupled food webs” (Lesser et al. 2020) and “Cross-habitat access modifies the ‘trophic relay’ in New England saltmarsh ecosystems” (Lesser et al. 2021).


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Locations: Sweeney (SW), West (WE), Clubhead (CL), and Mud South (MS)


Sampling Collection and Processing

16 mummichog were collected from each sampling creek via beach siene. All collected fish were frozen for later anaylsis. In the lab, each mummichog was weighed and measured. All fish were dried at 60°C for 48 hours and were then ground into a fine powder. Each ground sample was divided into two subsamples weighing between 0.10g - 0.15g. Each subsample was presurrized at 30 PSI and combusted in a stainless steel bomb and run in a Parr 6725 calorimeter attached to a Parr 6772 calorimetric thermometer. The two subsamples were then averaged together.



Version 1 March 10, 2022, original upload of data and metadata.  Used MarcrosExportEML_HTML (working)pie_excel2007_Jul2021.xlsm to check metadata.



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