uid=PIE,o=EDI,dc=edirepository,dc=org all public read LTE-TIDE-BrederTrap-GutContents Mummichog (Fundulus heteroclitus) gut content analysis from Breder trap transect collections in tidal creeks associated with long term fertilization experiments, Rowley, MA. Linda Deegan Woodwell Climate Research Center 508-444-1557 ldeegan@woodwellclimate.org http://orcid.org/0000-0002-1017-9599 James Nelson University of Louisiana at Lafayette (337) 482-6642 nelson@louisiana.edu https://www.nelsonecolab.net/ https://orcid.org/0000-0003-4607-3953 Plum Island Ecosystems LTER http://pie-lter.ecosystems.mbl.edu/ Justin Lesser justin.lesser@louisiana.edu associated researcher 2022 English
At PIE, mummichog (Fundulus heteroclitus) use the spring-cycle high tides to access the flooded high marsh platform and eat invertebrate prey, coupling the high marsh and aquatic creek food webs by gathering energy produced on the high marsh and making it available to the aquatic food web. Changes in the geomorphology of saltmarsh creek edges greatly influence the survival, biomass, and resource use of mummichog populations. Here we use gut content analysis assess the diet of mummichog on the high marsh platform during a flooding spring-cycle tide in July 2018 across 3 PIE creeks known to present different geomorphologic patterns in their low marsh zones. These data allow us to quantify the amount of terrestrial invertebrate prey mummichog consume on a single flooding tide and determine the impact altered low marsh geomorphology has on the trophic relationships in PIE food webs. These mummichog were captured in Breder traps; information about the consumer communities captured in these traps was recorded separately (LTE-TIDE-BrederTrap-Demographics). These data were included in part of the study “Habitat decoupling via saltmarsh creek geomorphology alters connection between spatially-coupled food webs” (Lesser et al. 2020) and were a portion of an MBL REU project.         
Disturbance Population Dynamics Core Areas population dynamics salt marshes invertebrates fishes nekton PIE LTER disturbance LTER Controlled Vocabulary
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https://pie-lter.ecosystems.mbl.edu/content/mummichog-fundulus-heteroclitus-gut-content-analysis-breder-trap-transect-collections-tidal Bounding box encompassing right and left branches of "Mud South" creek, off Mud Creek, Rowley, MA -70.850906372070 -70.846092224121 42.749408721924 42.746829986572 Bounding box encompassing left and right branches of West Creek, off Rowley River, Rowley, MA.   -70.851997375488 -70.846000671387 42.741001129150 42.736000061035 Bounding box encompassing right and left branches of Clubhead Creek off Rowley River, Rowley, MA. -70.842002868652 -70.835998535156 42.742000579834 42.737998962402 2018-07-27 2018-07-28 Version 1:  March 9, 2022, origianal upload of data and metadata.  Used MarcrosExportEML_HTML (working)pie_excel2007.xlsm 28/12 8:18 PM for QA/QC to EML 2.1.0     Plum Island Ecosystems LTER http://pie-lter.ecosystems.mbl.edu/ Plum Island Ecosystems LTER http://pie-lter.ecosystems.mbl.edu/ Plum Island Ecosystems
Locations: Mud South (MS), West (WE), and Clubhead (CL)  Breder Traps       In July of 2018, 8 stations of Breder traps were deployed per study creek, 4 stations in both the left and the right branch. Each station had 2 Breder traps: one was deployed in the low-marsh area, 1m from the TSA/SP boundary with the opening facing the creek in order to capture consumers as they moved onto the high-marsh area with the rising tide. The second was placed in the high-marsh area 1m from the TSA/SP boundary facing the marsh in order to capture consumers as they moved out of the high marsh as the tide receded. Breder traps were installed by clipping the grass and staking the trap into the ground to prevent any movement. The trap stations were placed with a minimum distance of 25m between them, ensuring independent samplings. Sampling was conducted over 2 nights, the 27th and 28th of July.   Sampling Collection and Processing   Traps were left out for 6 hours total, they were placed 3 hours before the incoming tide and collectred 3 hours after peak high tide.  When traps were collected all captured consumers were collected and frozen for analysis. In the lab, consumers were measured to the nearest millimeter and weighed. All weights were measured to the nearest 0.0001g. For fishes total length (TL) was measured from the tip of the snout to the end of the caudal fin. Mummichog individuals of a length equal or greater than 40mm were dissected, and stomach and intestine mass (stmass) was weighed, stomach and intestine content was then extracted and weighted (ctmass). Stomach contents were identified to general taxonomic categories, and an estimated percentage of total stomach content was visually assessed for each item (grid was used to assist estimation if needed, Hyslop 1980). Individuals with no identifiable aquatic or terrrestrial food material were not considered for analysis.  
LTE-TIDE-BrederTrap-GutContents_csv Mummichog (Fundulus heteroclitus) gut content analysis from Breder trap transect collections in tidal creeks associated with long term fertilization experiments, Rowley, MA.   LTE-TIDE-BrederTrap-GutContents_v1.csv 40108 8c19bb986db5d75c98e71dd5a290c111 e2d9cea2e5ea7479d4aa0861aa5a9ce9fd637f95 1 \r\n column , https://pie-lter.ecosystems.mbl.edu/sites/default/files/data/LTE-TIDE-BrederTrap-GutContents_v1.csv Date Date Date of collection date YYYY-MM-DD NA not available Creek Creek Creek Sampled string WE=West- Reference Geomorphology| CL=Clubhead- Altered Geomorphology MS=Mud South- Natural Geomorphology NA not available Branch Branch Branch Sampled string L=Left Branch R=Right Branch NA not available trap trap Trap number in each creek string Trap number in each creek Habitat Habitat Habitat along the marsh sampled string High= High marsh Low= Low marsh NA not available length length Animal length measured to the nearest millimeter millimeter real NA not available mass mass weight measured to the nearest 0.001 gram gram real NA not available stmass stmass Full stomach and intestine removed and weighed gram real NA not available ctmass ctmass Mass of content extracted form stomach and intestine gram real NA not available Amphipods Amphipods Number of amphipods found in gut number real NA not available Flies Flies Number of flies found in gut number real NA not available Shrimp Shrimp Number of shrimp material found in gut number real NA not available Beetles Beetles Number of beetles found in gut number real NA not available Snails Snails Number of snails found in gut number real NA not available Dragonflies Dragonflies Number of dragonflies found in gut number real NA not available Macro Invert Macro Invert Number of macro invertebrates found in gut number real NA not available Fish Fish Number of fish material found in gut number real NA not available Ants Ants Number of ants found in gut number real NA not available Ticks Ticks Number of ticks found in gut number real NA not available Spiders Spiders Number of spiders found in gut number real NA not available Worms Worms Number of worms found in gut number real NA not available Isopods Isopods Number of isopods found in gut number real NA not available percent Algae percent Algae Estimated percent of algae in stomach compared to total stomach content dimensionless real NA not available percent Plants percent Plants Estimated percent of plant material in stomach compared to total stomach content dimensionless real NA not available percent Protein percent Protein Estimated percent of proteinaceous material in stomach compared to total stomach content dimensionless real NA not available percent Unidentifiable Insects percent Unidentifiable Insects Estimated percent of unidentified insect material in stomach compared to total stomach content dimensionless real NA not available percent Amphipods percent Amphipods Estimated percentage of amphipods in stomach compared to total stomach content dimensionless real NA not available percent Flies percent Flies Estimated percentage of flies in stomach compared to total stomach content dimensionless real NA not available percent Shrimp percent Shrimp Estimated percentage of shrimp in stomach compared to total stomach content dimensionless real NA not available percent Beetles percent Beetles Estimated percentage of beetles in stomach compared to total stomach content dimensionless real NA not available percent Snails percent Snails Estimated percentage of snails in stomach compared to total stomach content dimensionless real NA not available percent Dragonflies percent Dragonflies Estimated percentage of dragonflies in stomach compared to total stomach content dimensionless real NA not available percent Macro Invert percent Macro Invert Estimated percentage of macro invertebrates in stomach compared to total stomach content dimensionless real NA not available percent Fish percent Fish Estimated percentage of fish in stomach compared to total stomach content dimensionless real NA not available percent Ants percent Ants Estimated percentage of ants in stomach compared to total stomach content dimensionless real NA not available percent Ticks percent Ticks Estimated percentage of ticks in stomach compared to total stomach content dimensionless real NA not available percent Spiders percent Spiders Estimated percentage of spiders in stomach compared to total stomach content dimensionless real NA not available percent Worms percent Worms Estimated percentage of worms in stomach compared to total stomach content dimensionless real NA not available percent Isopods percent Isopods Estimated percentage of isopods in stomach compared to total stomach content dimensionless real NA not available 360 LTE-TIDE-BrederTrap-GutContents_xlsx Excel metadata and data file associated with the csv data source file. Excel file after downloading is to be used for adding/editing new metadata and data.   LTE-TIDE-BrederTrap-GutContents_v1.xlsx 96800 092a81eeedf4ea84561c0cec5ea0fc12 18addacaf048b718f05e0c7b433beba3127338c9 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet https://pie-lter.ecosystems.mbl.edu/sites/default/files/data/LTE-TIDE-BrederTrap-GutContents_v1_0.xlsx document
.001 meters 0.001 kg a number a designation asserting the absence of an associated unit