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HTL-EST-Clam-chlorophyll
Water column chlorophyll concentrations associated with clam flat surveys during year 2010 in the Plum Island Sound estuary, Newbury, Rowley and Ipswich, MA.
Chuck
Hopkinson
Plum Island Ecosystems LTER
http://pie-lter.ecosystems.mbl.edu/
Plum Island Ecosystems LTER
http://pie-lter.ecosystems.mbl.edu/
Kyung-Ah
Koo
(706) 542-7671
kooka@uga.edu
associated researcher
Emily
Davenport
(706) 542-7671
davene@uga.edu
associated researcher
2015
English
Six to eight sites in the Plum Island Sound estuary , the mainstem of the Parker River and Rowley River, in areas close to where other clam samples were being taken for other studies, were selected for monthly chlorophyll sampling to determine the concentration of phytoplankton at these sites.
Primary Production
Core Areas
primary production
softshell clam
chlorophyll
PIE LTER
Plum Island Ecosystems
LTER Controlled Vocabulary
Data Policies
PIE LTER Data Use Agreement
This work is licensed under the Creative Commons Attribution 4.0 International License, CC BY - Attribution. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:
1) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier.
For example: Giblin, Anne; Hopkinson, Charles (2000): Rates of benthic metabolism and nutrient cycling in the Parker and Rowley Rivers of the Plum Island Sound estuary. Plum Island Ecosystems LTER, Long Term Ecological Research
Network. http://dx.doi.org/10.6073/pasta/17fa8b4fef50c7b85cac3d61482a7cb6
2) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. The Data User should contact the Data Set Contact to ensure the appropriate Granting Programs are acknowledged.
For example: Data sets were provided by the Plum Island Ecosystems LTER. This material is based upon funding supported by the National Science Foundation under Grants #OCE-9726921, OCE-0423565, OCE-1058747, OCE-1238212, OCE-1637630.
3) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
4) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.
5) Disclaimer. While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.
https://pie-lter.ecosystems.mbl.edu/content/water-column-chlorophyll-concentrations-associated-clam-flat-surveys-during-year-2010-plum-i
Site of clam surveys near Middle Road, north of Rte 1, Newbury MA
-70.898765563965
-70.898765563965
42.759368896484
42.759368896484
Site of clam surveys at confluence of Mill River with Parker River, Newbury, MA
-70.872085571289
-70.872085571289
42.755184173584
42.755184173584
Site of clam surveys near railroad bridge on Parker River, Newbury, MA
-70.863250732422
-70.863250732422
42.760368347168
42.760368347168
Site of clam surveys alias "House Boat" near the mouth of the Little River, Newbury, MA
-70.859016418457
-70.859016418457
42.762683868408
42.762683868408
Site of clam surveys, alias "Old Town Landing" near the Newbury marina, Newbury, MA
-70.838035583496
-70.838035583496
42.761283874512
42.761283874512
Site of clam surveys, alias "Grandkids Flat", southeast of Cape Merrill in Plum Island Sound, Newbury, MA
-70.810981750488
-70.810981750488
42.748298645020
42.748298645020
Site of clam surveys, end of Stackyard Rd, east of Nelson Island, in Plum Island Sound, Newbury, MA.
-70.821899414062
-70.821899414062
42.746250152588
42.746250152588
Site of clam surveys, Far Point, southeast of Cape Merrill in Plum Island Sound, Newbury MA
-70.799468994141
-70.799468994141
42.734500885010
42.734500885010
Site of clam surveys, near kite flag pole, mouth of Rowley River, Rowley, MA
-70.815147399902
-70.815147399902
42.727134704590
42.727134704590
Site of clam surveys, Third Creek, south of Roger Island River, Plum Island Sound, Ipswich, MA.
-70.816352844238
-70.816352844238
42.751800537109
42.751800537109
Site of clam surveys, Middle Ground, south of Roger Island River, Plum Island Sound Ipswich MA
-70.796669006348
-70.796669006348
42.719367980957
42.719367980957
Site of clam surveys, Ipswich Bluff flats, east of Ipswich Bay Yacht Club, Plum Island, Ipswich, MA.
-70.785896301270
-70.785896301270
42.708751678467
42.708751678467
Site of clam surveys, mouth of Clubhead Creek, off Rowley River, Rowley, MA
-70.838363647461
-70.838363647461
42.732017517090
42.732017517090
Site of clam surveys, mouth of Sweeney Creek, off Rowley River, Ipswich, MA
-70.847763061523
-70.847763061523
42.727844238281
42.727844238281
Site of clam surveys, tidal flat near PIE LTER Rowley field station, Rowley, MA.
-70.854949951172
-70.854949951172
42.725368499756
42.725368499756
2010-06-18
2010-11-29
Data complete for 2010 Version 01: September 29, 2015, data and metadata created to comply with importation to Drupal and LTER PASTA. Used MarcrosExportEML_HTML (working)pie_excel2007_Jan2015.xlsm 1/15/15 4:26 PM for QA/QC to EML 2.1.0
Plum Island Ecosystems LTER
http://pie-lter.ecosystems.mbl.edu/
Plum Island Ecosystems LTER
http://pie-lter.ecosystems.mbl.edu/
Plum Island Ecosystems
Six to eight sites in the Plum Island Sound estuary , the mainstem of the Parker River and Rowley River, in areas close to where other clam samples were being taken for other studies, were selected for monthly chlorophyll sampling to determine the concentration of phytoplankton at these sites. Roughly once a month, two 1L brown Nalgene bottles of water were sampled adjacent to each site from a boat at high tide. The water was kept cool and dark during transport to the lab, and filtered within three hours of collection. The procedure for filtration is outlined below: 1. Using square-ended forceps (to prevent poking a hole in the filter), place a single Whatman GF/F filter (2.5cm) onto a Gelman filter funnel. Make sure the funnel forms a tight seal with the base.2. The filter funnel should be attached to a vacuum system. We use a home-made PVC manifold that holds several funnels, and each funnel has a valve to open/close the vacuum. The manifold is attached to heavy-walled tygon tubing and a large vacuum flask (5 gallon glass water bottle). A second hose runs from the carboy to the vacuum pump. Use a moderate vacuum for the filtrations (ca. 100 to 200 mm Hg, or <7 in. Hg vac).3. Open the vacuum and pour in a pre-measured amount (using a clean graduated cylinder) of sample water. This is the tricky part. You may have to use trial and error to determine how much water you can filter before the filter clogs. Samples closer to the mouth of Plum Island Sound will require larger volumes (500-1000ml) than samples up the Parker River (150-700ml) depending upon season and discharge. Filter water samples in duplicate, be sure to record the total volume of water filtered for each duplicate sample.4. Using square-tipped forceps, gently fold the filter in half, with the side containing the sample on the inside of the fold. Remove the filter, keeping it folded, and place the filter between a small piece of aluminum foil 5. Label the outside of the aluminum foil (a square piece with dimensions of 5 x 5 cm) with the sample identification number. Use a black sharpie pen and make sure the ink dries (sharpie ink will destroy the pigment analysis….and the filters are magnets for sharpie ink!)6. Bend the edges of the foil to make sure the filter is sealed within the foil. Place the foil in a freezer (the colder the better). Keep the sample completely frozen and in the dark until you are ready to conduct the analysis To extract chlorophyll from the filters, the method outlined by the US EPA was followed: EPA Method 445.0, In Vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by FluorescenceElizabeth J. Arar and Gary B. Collins, Revision 1.2, September 1997 In short, the samples were placed in a test tube with 90% acetone and vortexed. They were kept cold overnight to allow time for the chlorophyll to be extracted, then vortexed again and analyzed on a Turner Trilogy Fluorometer. The fluorometer was calibrated with a chlorophyll standard curve using chlorophyll standards purchased from Turner Designs (standards had known concentrations and were then diluted to get a standard curve of 5 concentrations of chlorophyll ranging from low (0) to high (approx 200ug/L). After getting a chlorophyll reading, samples were acidified using 0.1NHCL to get the phaeophytin-a concentrations. Formulas for calculating chlorophyll-a and phaeophytin-a can be found in the Turner Trilogy Fluorometer user manual, page 36.
HTL-EST-Clam-chlorophyll_csv
Water column chlorophyll concentrations associated with clam flat surveys during year 2010 in the Plum Island Sound estuary, Newbury, Rowley and Ipswich, MA.
HTL-EST-Clam-chlorophyll.csv
6154
1d3812387d5415d0e9822554a13e4908
8329c31e04627b0114136bda820a390d5ea4053e
1
0
column
,
https://pie-lter.ecosystems.mbl.edu/sites/default/files/data/HTL-EST-Clam-chlorophyll.csv
Date
Date
Date of sampling
date
DD-MON-YYYY
NA
missing
Station_ID
Station_ID
Name of sampling station
string
Name of sampling station
Site_ID
Site_ID
Name of specific sampling site
string
Name of specific sampling site
Latitude
Latitude
Latitude of sampling station (decimal degrees)
degree
real
NA
missing
Longitude
Longitude
Longitude of sampling station (decimal degrees)
degree
real
NA
missing
Replicate
Replicate
Replicate of sample analyzed for chlorophyll
string
Replicate of sample analyzed for chlorophyll
Volume_filtered
Volume_filtered
volume of water filtered for chlorophyll analysis
milliliter
real
NA
missing
Chlorophyll-a
Chlorophyll-a
chlorophyll a concentration (ug/L)
microgramPerLiter
real
NA
missing
Phaeophytin-a
Phaeophytin-a
phaeophytin-a concentration (ug/L)
microgramPerLiter
real
NA
missing
HTL-EST-Clam-chlorophyll_xls
Excel metadata and data file associated with the csv data source file. Excel file after downloading is to be used for adding/editing new metadata and data.
HTL-EST-Clam-chlorophyll.xls
114688
be6923526565e046e02329c7b9159d8b
80f20f0d5085c955ecf388e87089303531c20cad
application/vnd.ms-excel
https://pie-lter.ecosystems.mbl.edu/sites/default/files/data/HTL-EST-Clam-chlorophyll.xls
document
360 degrees comprise a unit circle