uid=PIE,o=EDI,dc=edirepository,dc=org all public read HTL-EST-Clam-chlorophyll Water column chlorophyll concentrations associated with clam flat surveys during year 2010 in the Plum Island Sound estuary, Newbury, Rowley and Ipswich, MA. Chuck Hopkinson Plum Island Ecosystems LTER http://pie-lter.ecosystems.mbl.edu/ Plum Island Ecosystems LTER http://pie-lter.ecosystems.mbl.edu/ Kyung-Ah Koo (706) 542-7671 kooka@uga.edu associated researcher Emily Davenport (706) 542-7671 davene@uga.edu associated researcher 2015 English
Six to eight sites in the Plum Island Sound estuary , the mainstem of the Parker River and Rowley River, in areas close to where other clam samples were being taken for other studies, were selected for monthly chlorophyll sampling to determine the concentration of phytoplankton at these sites.
Primary Production Core Areas primary production softshell clam chlorophyll PIE LTER Plum Island Ecosystems LTER Controlled Vocabulary
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https://pie-lter.ecosystems.mbl.edu/content/water-column-chlorophyll-concentrations-associated-clam-flat-surveys-during-year-2010-plum-i Site of clam surveys near Middle Road, north of Rte 1, Newbury MA -70.898765563965 -70.898765563965 42.759368896484 42.759368896484 Site of clam surveys at confluence of Mill River with Parker River, Newbury, MA -70.872085571289 -70.872085571289 42.755184173584 42.755184173584 Site of clam surveys near railroad bridge on Parker River, Newbury, MA -70.863250732422 -70.863250732422 42.760368347168 42.760368347168 Site of clam surveys alias "House Boat" near the mouth of the Little River, Newbury, MA -70.859016418457 -70.859016418457 42.762683868408 42.762683868408 Site of clam surveys, alias "Old Town Landing" near the Newbury marina, Newbury, MA -70.838035583496 -70.838035583496 42.761283874512 42.761283874512 Site of clam surveys, alias "Grandkids Flat", southeast of Cape Merrill in Plum Island Sound, Newbury, MA -70.810981750488 -70.810981750488 42.748298645020 42.748298645020 Site of clam surveys, end of Stackyard Rd, east of Nelson Island, in Plum Island Sound, Newbury, MA. -70.821899414062 -70.821899414062 42.746250152588 42.746250152588 Site of clam surveys, Far Point, southeast of Cape Merrill in Plum Island Sound, Newbury MA -70.799468994141 -70.799468994141 42.734500885010 42.734500885010 Site of clam surveys, near kite flag pole, mouth of Rowley River, Rowley, MA -70.815147399902 -70.815147399902 42.727134704590 42.727134704590 Site of clam surveys, Third Creek, south of Roger Island River, Plum Island Sound, Ipswich, MA. -70.816352844238 -70.816352844238 42.751800537109 42.751800537109 Site of clam surveys, Middle Ground, south of Roger Island River, Plum Island Sound Ipswich MA -70.796669006348 -70.796669006348 42.719367980957 42.719367980957 Site of clam surveys, Ipswich Bluff flats, east of Ipswich Bay Yacht Club, Plum Island, Ipswich, MA. -70.785896301270 -70.785896301270 42.708751678467 42.708751678467 Site of clam surveys, mouth of Clubhead Creek, off Rowley River, Rowley, MA -70.838363647461 -70.838363647461 42.732017517090 42.732017517090 Site of clam surveys, mouth of Sweeney Creek, off Rowley River, Ipswich, MA -70.847763061523 -70.847763061523 42.727844238281 42.727844238281 Site of clam surveys, tidal flat near PIE LTER Rowley field station, Rowley, MA. -70.854949951172 -70.854949951172 42.725368499756 42.725368499756 2010-06-18 2010-11-29 Data complete for 2010 Version 01: September 29, 2015, data and metadata created to comply with importation to Drupal and LTER PASTA. Used MarcrosExportEML_HTML (working)pie_excel2007_Jan2015.xlsm 1/15/15 4:26 PM for QA/QC to EML 2.1.0 Plum Island Ecosystems LTER http://pie-lter.ecosystems.mbl.edu/ Plum Island Ecosystems LTER http://pie-lter.ecosystems.mbl.edu/ Plum Island Ecosystems
Six to eight sites in the Plum Island Sound estuary , the mainstem of the Parker River and Rowley River, in areas close to where other clam samples were being taken for other studies, were selected for monthly chlorophyll sampling to determine the concentration of phytoplankton at these sites. Roughly once a month, two 1L brown Nalgene bottles of water were sampled adjacent to each site from a boat at high tide. The water was kept cool and dark during transport to the lab, and filtered within three hours of collection. The procedure for filtration is outlined below: 1. Using square-ended forceps (to prevent poking a hole in the filter), place a single Whatman GF/F filter (2.5cm) onto a Gelman filter funnel. Make sure the funnel forms a tight seal with the base.2. The filter funnel should be attached to a vacuum system. We use a home-made PVC manifold that holds several funnels, and each funnel has a valve to open/close the vacuum. The manifold is attached to heavy-walled tygon tubing and a large vacuum flask (5 gallon glass water bottle). A second hose runs from the carboy to the vacuum pump. Use a moderate vacuum for the filtrations (ca. 100 to 200 mm Hg, or <7 in. Hg vac).3. Open the vacuum and pour in a pre-measured amount (using a clean graduated cylinder) of sample water. This is the tricky part. You may have to use trial and error to determine how much water you can filter before the filter clogs. Samples closer to the mouth of Plum Island Sound will require larger volumes (500-1000ml) than samples up the Parker River (150-700ml) depending upon season and discharge. Filter water samples in duplicate, be sure to record the total volume of water filtered for each duplicate sample.4. Using square-tipped forceps, gently fold the filter in half, with the side containing the sample on the inside of the fold. Remove the filter, keeping it folded, and place the filter between a small piece of aluminum foil 5. Label the outside of the aluminum foil (a square piece with dimensions of 5 x 5 cm) with the sample identification number. Use a black sharpie pen and make sure the ink dries (sharpie ink will destroy the pigment analysis….and the filters are magnets for sharpie ink!)6. Bend the edges of the foil to make sure the filter is sealed within the foil. Place the foil in a freezer (the colder the better). Keep the sample completely frozen and in the dark until you are ready to conduct the analysis To extract chlorophyll from the filters, the method outlined by the US EPA was followed: EPA Method 445.0, In Vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by FluorescenceElizabeth J. Arar and Gary B. Collins, Revision 1.2, September 1997 In short, the samples were placed in a test tube with 90% acetone and vortexed. They were kept cold overnight to allow time for the chlorophyll to be extracted, then vortexed again and analyzed on a Turner Trilogy Fluorometer. The fluorometer was calibrated with a chlorophyll standard curve using chlorophyll standards purchased from Turner Designs (standards had known concentrations and were then diluted to get a standard curve of 5 concentrations of chlorophyll ranging from low (0) to high (approx 200ug/L). After getting a chlorophyll reading, samples were acidified using 0.1NHCL to get the phaeophytin-a concentrations. Formulas for calculating chlorophyll-a and phaeophytin-a can be found in the Turner Trilogy Fluorometer user manual, page 36.
HTL-EST-Clam-chlorophyll_csv Water column chlorophyll concentrations associated with clam flat surveys during year 2010 in the Plum Island Sound estuary, Newbury, Rowley and Ipswich, MA. HTL-EST-Clam-chlorophyll.csv 6154 1d3812387d5415d0e9822554a13e4908 8329c31e04627b0114136bda820a390d5ea4053e 1 0 column , https://pie-lter.ecosystems.mbl.edu/sites/default/files/data/HTL-EST-Clam-chlorophyll.csv Date Date Date of sampling date DD-MON-YYYY NA missing Station_ID Station_ID Name of sampling station string Name of sampling station Site_ID Site_ID Name of specific sampling site string Name of specific sampling site Latitude Latitude Latitude of sampling station (decimal degrees) degree real NA missing Longitude Longitude Longitude of sampling station (decimal degrees) degree real NA missing Replicate Replicate Replicate of sample analyzed for chlorophyll string Replicate of sample analyzed for chlorophyll Volume_filtered Volume_filtered volume of water filtered for chlorophyll analysis milliliter real NA missing Chlorophyll-a Chlorophyll-a chlorophyll a concentration (ug/L) microgramPerLiter real NA missing Phaeophytin-a Phaeophytin-a phaeophytin-a concentration (ug/L) microgramPerLiter real NA missing HTL-EST-Clam-chlorophyll_xls Excel metadata and data file associated with the csv data source file. Excel file after downloading is to be used for adding/editing new metadata and data. HTL-EST-Clam-chlorophyll.xls 114688 be6923526565e046e02329c7b9159d8b 80f20f0d5085c955ecf388e87089303531c20cad application/vnd.ms-excel https://pie-lter.ecosystems.mbl.edu/sites/default/files/data/HTL-EST-Clam-chlorophyll.xls document
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