We measured chlorophyll concentrations in the surface waters and sediments of six ponds in three regions of the PIE-LTER marshes during summer 2016. The goal was to assess whether pond microalgal community abundances varied predictably with pond dimensions (e.g., surface area, volume) or geographic attributes (e.g., elevation, distance from upland, marsh region). Samples were collected from several locations in each pond in order to capture spatial heterogeneity.
Surface water was collected for chlorophyll from two locations in each pond into 1 L combusted (450 °C) glass bottles and filtered through combusted and pre-weighed glass fiber filters. Benthic microalgae were collected from all ponds by placing four replicate, combusted glass slides on surface sediments and then scraping attached material onto combusted glass fiber filters. Surface water and sediment chlorophyll were analyzed according to Neubauer et al. .
Neubauer, S. C., W. D. Miller, and I. C. Anderson (2000), Carbon cycling in a tidal freshwater marsh ecosystem: a carbon gas flux study, Marine Ecology Progress Series 199, 13-30.
Version 01: August 17, 2020, data and metadata updates to comply with importation to DEIMS7 and LTER Data Portal. Used MarcrosExportEML_HTML (working)pie_excel2007_Sep2019.xlsm 9/3/19 5:19 PM for QA/QC to EML 2.1.0.