Melampus bidentatus (coffee bean snail) length and biomass measurements for Rowley River tidal creeks associated with long term fertilization experiments, Rowley and Ipswich, MA. The TIDE project aims to simulate eutrophication on a large scale by the addition of NO3- aiming to reach 70μM concentrations from May to September every year during the growing season. This fertilization of the marsh has been going on at Sweeney Creek since the 2004 growing season through 2016 and at Clubhead Creek in 2005 and from 2009 till 2016. Invertebrates were sampled after fertilization ended to look at recovery.
All samples taken in summer no sooner than late June and no later than mid-September. In the field, 10 quadrats (0.01 m2) (in either Spartina patens or stunted Spartina alterniflroa habitats) of each creek branch. Data can be paired with high-marsh invertebrate density file. For 2010/2009 data, only 10 of randomly selected snails in a quadrat were measured for shell length. Shell-free biomass determined using allometric equation in Johnson 2011 Marine Ecology Progress Series 438:143-152.
Reference creeks are West and Nelson
Nutrient Enriched creeks are Sweeney and Clubhead. Sweeney has been fertilized every summer from 2004-2016 . Clubhead had been fertilized in 2005 and then from 2009-2016.
All creeks have two branches determined by direction at confluence while facing upstream (Left or Right). Samples were not collected in West-Left after 2019 due to restricted access.
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